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验证数据展示

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB result of IKZF1 antibody (66966-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKZF1 transfected MOLT-4 cells. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IKZF1 Monoclonal antibody, PBS Only

IKZF1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66966-1-PBS

产品说明书

CloneNo. 1D6B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig, rabbit

应用

WB, Indirect ELISA

别名

DNA binding protein Ikaros, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66966-1-PBS targets IKZF1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, pig, rabbit
免疫原

CatNo: Ag28637

Product name: Recombinant human IKZF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-303 aa of BC018349

Sequence: MDADEGQDMSQVSGKESPPVSDTPDEGDEPMPIPEDLSTTSGGQQSSKSDRVVASNVKVETQSDEENGRACEMNGEECAEDLRMLDASGEKMNGSHRDQGSSALSGVGGIRLPNGKLKCDICGIICIGPNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHSGEKPFKCHLCNYACRRRDALTGHLRTHSVIKEETNHSEMAEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVIS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IKAROS family zinc finger 1 (Ikaros)
别名 DNA binding protein Ikaros, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1
计算分子量 477 aa, 53 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC018349
基因名称 IKZF1
Gene ID (NCBI) 10320
RRIDAB_2882289
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13422
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.