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  • Featured Product
  • KD/KO Validated

IKZF1 Monoclonal antibody, PBS Only

IKZF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66966-1-PBS

Print datasheet

Synonyms

IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros

验证数据展示

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB result of IKZF1 antibody (66966-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKZF1 transfected MOLT-4 cells. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

产品信息

66966-1-PBS targets IKZF1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig, rabbit
Immunogen IKZF1 fusion protein Ag28637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IKAROS family zinc finger 1 (Ikaros)
Synonyms IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC018349
Gene Symbol IKZF1
Gene ID (NCBI) 10320
RRIDAB_2882289
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13422
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.