• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

    WB result of IKZF1 antibody (66966-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKZF1 transfected MOLT-4 cells. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IKZF1 Monoclonal antibody, PBS Only

IKZF1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66966-1-PBS

产品说明书

CloneNo. 1D6B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig, rabbit

应用

WB, Indirect ELISA

别名

IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66966-1-PBS targets IKZF1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, pig, rabbit
免疫原 IKZF1 fusion protein Ag28637 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IKAROS family zinc finger 1 (Ikaros)
别名 IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros
计算分子量 477 aa, 53 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC018349
基因名称 IKZF1
Gene ID (NCBI) 10320
RRIDAB_2882289
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13422
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.