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IKZF1 Monoclonal antibody

IKZF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rabbit, Pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66966-1-Ig

Print datasheet

Synonyms

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

验证数据展示

  • WB analysis using 66966-1-Ig

    WB analysis using 66966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit spleen using 66966-1-Ig

    WB analysis of rabbit spleen using 66966-1-Ig

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 66966-1-Ig

    IHC staining of human lymphoma using 66966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of pig thymus using 66966-1-Ig

    WB analysis of pig thymus using 66966-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66966-1-Ig

    WB analysis using 66966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, pig thymus tissue, rabbit spleen tissue, Ramos cells, Daudi cells, Sp2/0 cells, MOLT-4 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66966-1-Ig targets IKZF1 in WB, ELISA applications and shows reactivity with Human, mouse, Rabbit, Pig samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, Rabbit, Pig
Cited Reactivityhuman
Immunogen IKZF1 fusion protein Ag28637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IKAROS family zinc finger 1 (Ikaros)
Synonyms DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC018349
Gene Symbol IKZF1
Gene ID (NCBI) 10320
RRIDAB_2882289
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13422
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

实验方案

Product Specific Protocols
WB protocol for IKZF1 antibody 66966-1-IgDownload protocol
IHC protocol for IKZF1 antibody 66966-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2.

Authors - Elanora Dovat
View Article