验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Jurkat cells, MOLT-4 cells, pig thymus tissue, rabbit spleen tissue, Ramos cells, Daudi cells, Sp2/0 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
66966-1-Ig targets IKZF1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, pig, rabbit |
| 文献引用反应性 | human |
| 免疫原 | IKZF1 fusion protein Ag28637 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IKAROS family zinc finger 1 (Ikaros) |
| 别名 | IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros |
| 计算分子量 | 477 aa, 53 kDa |
| 观测分子量 | 55-65 kDa |
| GenBank蛋白编号 | BC018349 |
| 基因名称 | IKZF1 |
| Gene ID (NCBI) | 10320 |
| RRID | AB_2882289 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13422 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IKZF1 antibody 66966-1-Ig | Download protocol |
| IHC protocol for IKZF1 antibody 66966-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun YTHDF2 upregulation and subcellular localization dictate CD8 T cell polyfunctionality in anti-tumor immunity | ||
Int J Mol Sci Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2. |