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CoraLite® Plus 488-conjugated IKZF1 Monoclonal antibody

IKZF1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6B5

Cat no : CL488-66966

Print datasheet

Synonyms

IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros

验证数据展示

  • IF Staining of Jurkat using CL488-66966

    IF Staining of Jurkat using CL488-66966

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 IKZF1 antibody (CL488-66966, Clone: 1D6B5 ) at dilution of 1:200.

  • FC experiment of human PBMCs using CL488-66966

    FC experiment of human PBMCs using CL488-66966

    1x10^6 human PBMCs were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IKZF1 Monoclonal antibody (CL488-66966, Clone:1D6B5), and 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 66966-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66966 targets IKZF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, pig, rabbit
Immunogen IKZF1 fusion protein Ag28637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IKAROS family zinc finger 1 (Ikaros)
Synonyms IKAROS, IK1, Hs.54452, hIk 1, DNA-binding protein Ikaros
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC018349
Gene Symbol IKZF1
Gene ID (NCBI) 10320
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13422
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IKZF1 antibody CL488-66966Download protocol
FC protocol for CL Plus 488 IKZF1 antibody CL488-66966Download protocol
Standard Protocols
Click here to view our Standard Protocols