CoraLite®555-conjugated LBR Polyclonal antibody
LBR Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12398-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-12398 targets LBR in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | LBR fusion protein Ag3088 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lamin B receptor |
| Synonyms | DHCR14B, FLJ43126, lamin B receptor, LBR, LMN2R, PHA |
| Calculated Molecular Weight | 615 aa, 71 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC020079 |
| Gene Symbol | LBR |
| Gene ID (NCBI) | 3930 |
| RRID | AB_2919610 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14739 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 LBR antibody CL555-12398 | Download protocol |
| FC protocol for CL555 LBR antibody CL555-12398 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
