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验证数据展示

  • IF Staining of HepG2 using CL488-11128

    IF Staining of HepG2 using CL488-11128

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MYH9 antibody (CL488-11128) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for IF/ICC
Cat No. CL488-11128

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11128-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11128 targets MYH9 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1612

Product name: Recombinant human MYH9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1741-1960 aa of BC011915

Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 9, non-muscle
别名 DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9
计算分子量 227 kDa
观测分子量 224 kDa
GenBank蛋白编号BC011915
基因名称 MYH9
Gene ID (NCBI) 4627
RRIDAB_3672482
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35579
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYH9 antibody CL488-11128Download protocol
Standard Protocols
Click here to view our Standard Protocols