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CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8D3

Cat no : CL488-66360-1

Print datasheet

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

验证数据展示

  • FC experiment of human PBMCs using CL488-66360-1

    FC experiment of human PBMCs using CL488-66360-1

    1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of A431 using CL488-66360-1

    FC experiment of A431 using CL488-66360-1

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inhuman PBMCs, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 66360-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.

Tested Applications FC Application Description
Tested Reactivity Megathura crenulata
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IgG1 Isotype Control
Synonyms Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IgG1 Isotype Control antibody CL488-66360-1Download protocol
Standard Protocols
Click here to view our Standard Protocols