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CoraLite®594-conjugated NFATC2 Polyclonal antibody
NFATC2 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | U-251 cells |
Positive FC (Intra) detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy NFATC2 Ready-To-Use IHC Kit. NFATC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22023-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-22023 targets NFATC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | NFATC2 fusion protein Ag16849 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 |
Synonyms | NFAT1, NFATP, NF-ATc2, NFAT pre-existing subunit, NFAT pre existing subunit |
Calculated Molecular Weight | 925 aa, 100 kDa |
Observed Molecular Weight | 135 kDa |
GenBank Accession Number | BC136418 |
Gene Symbol | NFATC2 |
Gene ID (NCBI) | 4773 |
RRID | AB_2919868 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13469 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 NFATC2 antibody CL594-22023 | Download protocol |
FC protocol for CL594 NFATC2 antibody CL594-22023 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |