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ChromoTek Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119]

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

Applications

IF, WB, FC

Conjugate

2x Cys

Host

Alpaca

Reactivity

Rabbit

Cat No : srb2GCys2-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation Data Gallery

  • Dot blot assay demonstrates that Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] has no cross-reactivity to mouse, rat, or human immunoglobulins.

    Dot blot assay demonstrates that Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] has no cross-reactivity to mouse, rat, or human immunoglobulins.

  • The affinity of Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] towards rabbit IgG was determined using biolayer interferometry (BLI). Biotinylated, polyclonal rabbit IgG was immobilized on Streptavidin biosensors and assayed with 0.3 to 10 nM VHH. Fit indicates a 1:1 binding model fitted to the data. 

    The affinity of Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] towards rabbit IgG was determined using biolayer interferometry (BLI). Biotinylated, polyclonal rabbit IgG was immobilized on Streptavidin biosensors and assayed with 0.3 to 10 nM VHH. Fit indicates a 1:1 binding model fitted to the data. 

Product Information

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

ApplicationsIF, WB, FC
ClonalityMonoclonal Nanobody
CloneCTK0119
HostAlpaca
Affinity0.2 nM
Specificity/TargetFc-fragment of Rabbit IgG
ReactivityRabbit
ConjugateN- and C-terminal cysteine conjugation with thiol-reactive reagents
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide
Storage ConditionShipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C.
RRIDAB_3094643