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ChromoTek Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH CoraLite® Plus 647 [CTK0119, CTK0120]

Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

Applications

WB, IF

Conjugate

CoraLite® Plus 647

Host

Alpaca

Reactivity

Rabbit IgG

Cat No : srb2GCL647-1

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation Data Gallery

  • HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 647 (srb2GCL647-1), 1:500.

    HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 647 (srb2GCL647-1), 1:500.

  • Immunofluorescence analysis of A431 cells stained with rabbit anti-COXIV antibody (11242-1-AP, magenta) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 647 (srb2GCL647-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells stained with rabbit anti-COXIV antibody (11242-1-AP, magenta) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 647 (srb2GCL647-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Dot blot assay demonstrates that both clones contributing to Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH CoraLite Plus 647 [CTK0119, CTK0120] have no cross-reactivity to mouse, rat, or human immunoglobulins.

    Dot blot assay demonstrates that both clones contributing to Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH CoraLite Plus 647 [CTK0119, CTK0120] have no cross-reactivity to mouse, rat, or human immunoglobulins.

Product Information

Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-rabbit IgG, recombinant VHH reagent uses a novel class of rabbit IgG-specific antibodies. This secondary antibody product consists of nanobodies that bind to rabbit IgG with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, see details below.
ApplicationsWB, IF
HostAlpaca
Species ReactivityReactivity: Rabbit IgG
No cross-reactivity: mouse, rat and human IgGs.
Target/SpecificityRabbit IgG
Physical StateLiquid
ConjugateCoraLite® Plus 647
Suggested DilutionImmunofluorescence 1:500
Western blot: 1:500
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to mouse, rat or human IgGs
ImmunogenPurified rabbit IgG
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0119 (VHH0708), CTK0120 (VHH0953)
Affinity (KD)CTK0119: KD = 0.2 nM, CTK120: KD = <10 pM
RRIDAB_3094641
Excitation / EmissionExcitation max: 654 nm, Emission max: 674 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use