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验证数据展示

  • IF Staining of mouse eye using CL488-12833

    IF Staining of mouse eye using CL488-12833

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CoraLite® Plus 488 Neuropeptide Y antibody (CL488-12833) at dilution of 1:200.

CoraLite® Plus 488-conjugated Neuropeptide Y Polyclonal antibody

Neuropeptide Y Polyclonal Antibody for IF-P
Cat No. CL488-12833

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

CPON, Neuropeptide tyrosine, neuropeptide Y, NPY, PYY4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse eye tissue
For other applications, we recommend the unconjugated version of this antibody, 12833-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12833 targets Neuropeptide Y in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Neuropeptide Y fusion protein Ag3568 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuropeptide Y
Synonyms CPON, Neuropeptide tyrosine, neuropeptide Y, NPY, PYY4
Calculated Molecular Weight 97 aa, 11 kDa
GenBank Accession NumberBC029497
Gene Symbol Neuropeptide Y
Gene ID (NCBI) 4852
RRIDAB_3083932
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01303
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide Y (NPY) is one of the most abundant peptides in the mammalian central nervous system (CNS). NPY has been linked to several physiological and pathological functions, such as feeding behaviour, memory processing, pain, anxiety, cell proliferation and many other processes in the central and peripheral nervous systems. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. In addition, NPY was suggested as a potential neuroprotective agent in Alzheimer's disease by counteracting the toxic effect of β-amyloid in an in vitro model.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Neuropeptide Y antibody CL488-12833Download protocol
Standard Protocols
Click here to view our Standard Protocols