验证数据展示
产品信息
66378-1-PBS targets Occludin as part of a matched antibody pair:
MP51160-1: 66378-2-PBS capture and 66378-1-PBS detection (validated in Cytometric bead array)
MP51160-3: 66378-1-PBS capture and 66378-4-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Applications | WB, IHC, Cytometric bead array, Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | Occludin fusion protein Ag4057 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | occludin |
Synonyms | OCLN, 1D3C4 |
Calculated Molecular Weight | 522 aa, 59 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC029886 |
Gene Symbol | Occludin |
Gene ID (NCBI) | 4950 |
RRID | AB_2881755 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q16625 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).