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PAX8 Recombinant antibody, PBS Only

PAX8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B14

Cat no : 80756-1-PBS

Print datasheet

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

验证数据展示

  • WB analysis using 80756-1-RR (same clone as 80756-1-PBS)

    WB analysis using 80756-1-RR (same clone as 80756-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80756-1-RR (PAX8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX8 antibody included
  • IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX8 antibody included
  • IF Staining of SKOV-3 using 80756-1-RR (same clone as 80756-1-PBS)

    IF Staining of SKOV-3 using 80756-1-RR (same clone as 80756-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (80756-1-RR, Clone: 6B14 ) at dilution of 1:1300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80756-1-PBS

    Affinity and Kinetic Characterization of 80756-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80756-1-RR against Human PAX8 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

产品信息

80756-1-PBS targets PAX8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa, 58 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.