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PAX8 Recombinant antibody

PAX8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6B14

Cat no : 80756-1-RR

Print datasheet

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

验证数据展示

  • WB analysis using 80756-1-RR

    WB analysis using 80756-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80756-1-RR (PAX8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 80756-1-RR

    IHC staining of human ovary tumor using 80756-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included
  • IHC staining of human ovary tumor using 80756-1-RR

    IHC staining of human ovary tumor using 80756-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included
  • IF Staining of SKOV-3 using 80756-1-RR

    IF Staining of SKOV-3 using 80756-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (80756-1-RR, Clone: 6B14 ) at dilution of 1:1300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80756-1-RR

    Affinity and Kinetic Characterization of 80756-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80756-1-RR against Human PAX8 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman ovary tumor tissue, mouse kidney tissue, human renal cell carcinoma tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80756-1-RR targets PAX8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa, 58 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.

实验方案

Product Specific Protocols
WB protocol for PAX8 antibody 80756-1-RRDownload protocol
IHC protocol for PAX8 antibody 80756-1-RRDownload protocol
IF protocol for PAX8 antibody 80756-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols