CoraLite® Plus 488-conjugated Phospho-Caspase 9 (Ser196) Recombinant antibody
Phospho-Caspase 9 (Ser196) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3P16
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 80346-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80346 targets Phospho-Caspase 9 (Ser196) in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Synonyms | CASP9, Caspase 9, Caspase 9/p35/p10, Apoptotic protease Mch-6, Apoptotic protease Mch 6 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa, 35 kDa |
| GenBank Accession Number | BC002452 |
| Gene Symbol | Caspase 9 |
| Gene ID (NCBI) | 842 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55211 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46kDa precursor protein which can be cleaved into a 35kDa subunit and a 11kDa subunit. The phosphorylated type can be detected at 55kDa and 35kDa. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It can be phosphorylated by PKB/AKT1 at Ser196, this modification will downregulate its activity and decrease apoptosis. Akt phosphorylation site found in human caspase 9 is absent in mouse caspase 9.It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507)