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验证数据展示

  • FC experiment of HeLa using CL488-67898

    FC experiment of HeLa using CL488-67898

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-S6 Ribosomal protein (Ser235) (CL488-67898, Clone:2A4B6), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HeLa using CL488-67898

    IF Staining of HeLa using CL488-67898

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-S6 Ribosomal protein (Ser235) antibody (CL488-67898, Clone: 2A4B6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody

Phospho-S6 Ribosomal protein (Ser235) Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67898

产品说明书

CloneNo. 2A4B6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

p S6 Ribosomal protein Ser235, p S6 Ribosomal protein (Ser235), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser235)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67898-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67898 targets Phospho-S6 Ribosomal protein (Ser235) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S6
别名 p S6 Ribosomal protein Ser235, p S6 Ribosomal protein (Ser235), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser235)
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
RRIDAB_2934584
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-S6 Ribosomal protein (Ser235) antibody CL488-67898Download protocol
Standard Protocols
Click here to view our Standard Protocols