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验证数据展示

  • IF Staining of mouse heart using CL594-67469

    IF Staining of mouse heart using CL594-67469

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 SF3B3 antibody (CL594-67469, Clone: 2H7F5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using CL594-67469

    IF Staining of HepG2 using CL594-67469

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SF3B3 antibody (CL594-67469, Clone: 2H7F5 ) at dilution of 1:200.

CoraLite®594-conjugated SF3B3 Monoclonal antibody

SF3B3 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL594-67469

产品说明书

CloneNo. 2H7F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SF3B3 Ready-To-Use IHC Kit. SF3B3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67469-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67469 targets SF3B3 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 SF3B3 fusion protein Ag6315 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 splicing factor 3b, subunit 3, 130kDa
别名 SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017
计算分子量 136 kDa
观测分子量 130-135 kDa
GenBank蛋白编号BC003146
基因名称 SF3B3
Gene ID (NCBI) 23450
RRIDAB_2920141
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15393
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

实验方案

Product Specific Protocols
IF protocol for CL594 SF3B3 antibody CL594-67469Download protocol
Standard Protocols
Click here to view our Standard Protocols