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ChromoTek Strep-NanoTrap Agarose Kit

The ChromoTek Strep-NanoTrap Agarose Kit consists of an anti-Strep-tag® Nanobody/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Strep-tagged proteins from cell extracts of various organisms.

Specificity

Strep-Tag® (sequence: SAWSHPQFEK) and Twin-Strep-Tag® (sequence: SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Cat No : qtak

Synonyms

Strep Trap, SAWSHPQFEK-Trap, Strep-tag-Trap

Validation Data Gallery

  • IP of StrepTag-Aurora Kinase B fusion protein from transfected HEK293T cells using either Strep-NanoTrap Agarose (left) or a Competitor Resin (right). The Strep-NanoTrap binds with higher affinity to the strep-tagged protein in comparison to the competitor product. I: Input, F: Flow-Through, B: Bound.

    IP of StrepTag-Aurora Kinase B fusion protein from transfected HEK293T cells using either Strep-NanoTrap Agarose (left) or a Competitor Resin (right). The Strep-NanoTrap binds with higher affinity to the strep-tagged protein in comparison to the competitor product. I: Input, F: Flow-Through, B: Bound.

  • Co-IP using Strep-NanoTrap Agarose followed by multiplexed WB of StrepTag-AurKinB, CDC37, and HSP090AB1 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with StrepTag-Aurora Kinase B construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. For the WB analysis, StrepTag was detected using an Anti-StrepTag DY-649 antibody, CDC37 was detected with an Anti-CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FlexAble CoraLite Plus 750 Antibody Labeling Kit for Mouse IgG2a (KFA044), and HSP90AB1 was detected using an Anti-HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Mouse IgG2b (KFA061).

    Co-IP using Strep-NanoTrap Agarose followed by multiplexed WB of StrepTag-AurKinB, CDC37, and HSP090AB1 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with StrepTag-Aurora Kinase B construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. For the WB analysis, StrepTag was detected using an Anti-StrepTag DY-649 antibody, CDC37 was detected with an Anti-CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FlexAble CoraLite Plus 750 Antibody Labeling Kit for Mouse IgG2a (KFA044), and HSP90AB1 was detected using an Anti-HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Mouse IgG2b (KFA061).

  • IP followed by WB of either N- (left) or C- (right) terminally-linked TwinStrepTag-Aurora Kinase B fusion proteins from transfected HEK293T cells using either Strep-NanoTrap Agarose or a Competitor Resin. The Strep-NanoTrap binds to both N- and C-terminally linked TwinStrepTag proteins with higher affinity than the competitor product. WB analysis additionally indicates that all TwinStrepTagged material is sucessfully eluted in the Bound (B) fraction, with little to no material left in the Flow-Through (F).

    IP followed by WB of either N- (left) or C- (right) terminally-linked TwinStrepTag-Aurora Kinase B fusion proteins from transfected HEK293T cells using either Strep-NanoTrap Agarose or a Competitor Resin. The Strep-NanoTrap binds to both N- and C-terminally linked TwinStrepTag proteins with higher affinity than the competitor product. WB analysis additionally indicates that all TwinStrepTagged material is sucessfully eluted in the Bound (B) fraction, with little to no material left in the Flow-Through (F).

Product Information

The ChromoTek Strep-NanoTrap Agarose Kit consists of an anti-Strep-tag® Nanobody/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Strep-tagged proteins from cell extracts of various organisms.

DescriptionThe Strep-Trap Agarose Kit contains Strep-Trap agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Strep-tagged proteins.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the peptide sequence SAWSHPQFEK, also known as
Strep-Tag® or Strep-TagII®, fused to a protein of interest at N- or C-terminal position. In addition, this trap binds to the peptide sequence SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK, also known as
Twin-Strep-Tag®, fused to a protein of interest at N- or C-terminal position.
Binding Capacity25 ug of recombinant SAWSHPQFEK-tagged protein (~30 kDa) per 25 uL bead slurry
ConjugateAgarose beads; ~90 um (cross-linked 4% agarose beads)
Elution buffer2x SDS-sample buffer (Lammli)
Wash Buffer Compatibility1M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 2-3 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 480 nM for N-terminal Strep-Tag® and 600 nM for C-terminal Strep-Tag®
Compatibility with mass spectrometryThe Strep-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3107051
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component Description
Strep-NanoTrap Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution