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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66777-1-Ig (same clone as 66777-1-PBS)

    WB analysis using 66777-1-Ig (same clone as 66777-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66777-1-Ig (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOMM20 antibody included
  • IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

    IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66777-1-Ig (same clone as 66777-1-PBS)

    FC experiment of HeLa using 66777-1-Ig (same clone as 66777-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TOM20 (66777-1-Ig, Clone:1D6F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

TOM20 Monoclonal antibody, PBS Only

TOM20 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66777-1-PBS

产品说明书

CloneNo. 1D6F5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TOMM20, TOMM 20, tom 20, MOM19, MAS20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TOMM20 Ready-To-Use IHC Kit. TOMM20 primary antibody included.

产品信息

66777-1-PBS targets TOM20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TOM20 fusion protein Ag2378 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 20 homolog (yeast)
别名 TOMM20, TOMM 20, tom 20, MOM19, MAS20
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000882
基因名称 TOM20
Gene ID (NCBI) 9804
RRIDAB_2882123
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15388
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.