ChromoTek V5-Trap® Agarose Kit

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat No : v5tak

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

DescriptionThe V5-Trap Agarose Kit contains V5-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Effective elution with V5-peptide

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2868497
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
V5-Trap™ Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate

Publications

ApplicationTitle

PLoS One

PSMD11 modulates circadian clock function through PER and CRY nuclear translocation

Authors - Sibel Cal-Kayitmazbatir