ChromoTek V5-Trap® Magnetic Agarose Kit
The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.
Specificity
V5-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat No : v5tmak
Synonyms
Validation Data Gallery
Product Information
The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 40 nM |
Specificity/Target | V5-Tag |
Coupled VHH | recombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment |
Conjugate | Magnetic Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant V5-tagged protein ~30 kDa |
Elution buffer | V5-peptide. For details see below. SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Kit components
Component | Description |
---|---|
V5-Trap™ Magnetic Agarose | 20 reactions (500 µL) |
Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
Dilution buffer | Dilution of cell lysate |
Documentation
SDS |
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SDS V5-Trap Magnetic Agarose Kit (PDF) |
Protocol |
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Protocol V5-Trap Magnetic Agarose Kit (PDF) |
Troubleshooting |
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Troubleshooting guide immunoprecipitation (PDF) |