• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0

ChromoTek V5-Trap® Magnetic Agarose Kit

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat No : v5tmak

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

Validation Data Gallery

  • V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

    V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

  • Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

    Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

  • V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

    V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

  • Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

    Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

Product Information

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-Tag
Coupled VHHrecombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant V5-tagged protein ~30 kDa
Elution bufferV5-peptide. For details see below.
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
V5-Trap™ Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate