验证数据展示
产品信息
80232-1-PBS targets Vimentin in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IF-Fro, Indirect ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Vimentin fusion protein Ag0489 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | vimentin |
别名 | VIM, 6K21 |
计算分子量 | 466 aa, 54 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC000163 |
基因名称 | VIM |
Gene ID (NCBI) | 7431 |
ENSEMBL Gene ID | ENSG00000026025 |
RRID | AB_2918879 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08670 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).