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  • Featured Product
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Vimentin Recombinant antibody

Vimentin Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

6K21

Cat No : 80232-1-RR

Print datasheet

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

验证数据展示

  • WB analysis using 80232-1-RR

    WB analysis using 80232-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 80232-1-RR

    WB analysis of A549 using 80232-1-RR

    WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells.

  • IHC staining of human breast cancer using 80232-1-RR

    IHC staining of human breast cancer using 80232-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 80232-1-RR

    IF Staining of HepG2 using 80232-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, U-251 cells, A549 cells, HeLa cells, U2OS cells, NIH/3T3 cells, 4T1 cells, ROS1728 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1250-1:5000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80232-1-RR targets Vimentin in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vimentin
Synonyms Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2918879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

实验方案

Product Specific Protocols
WB protocol for Vimentin antibody 80232-1-RRDownload protocol
IHC protocol for Vimentin antibody 80232-1-RRDownload protocol
IF protocol for Vimentin antibody 80232-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols