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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Vimentin Recombinant antibody

Vimentin Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

6K21

Cat No : CL647-80232

Print datasheet

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

验证数据展示

  • IF Staining of HeLa using CL647-80232

    IF Staining of HeLa using CL647-80232

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 Vimentin antibody (CL647-80232, Clone: 6K21 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL647-80232

    IF Staining of HepG2 using CL647-80232

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 Vimentin antibody (CL647-80232, Clone: 6K21 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80232-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-80232 targets Vimentin in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vimentin
Synonyms Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Vimentin antibody CL647-80232Download protocol
Standard Protocols
Click here to view our Standard Protocols