验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-80232 targets Vimentin in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | Vimentin fusion protein Ag0489 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | vimentin |
别名 | Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin |
计算分子量 | 466 aa, 54 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC000163 |
基因名称 | VIM |
Gene ID (NCBI) | 7431 |
ENSEMBL Gene ID | ENSG00000026025 |
RRID | AB_2923957 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08670 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 Vimentin antibody CL647-80232 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |