Validation Data Gallery
Product Information
Nano-Secondary® anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB |
| Host | Alpaca |
| Species Reactivity | Mouse IgG2b Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3 |
| Target/Specificity | This Nanobody mixture recognizes the Fc fragment of mouse IgG2b. |
| Physical State | Liquid |
| Conjugate | Alexa Fluor® 488 |
| Suggested Dilution | Immunofluorescence 1:1,000 Western blot 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Mixture of 2 monoclonal Nanobodies |
| Class | Recombinant |
| Format | Alpaca single domain antibodies, monovalent |
| Cross-reactivity | No cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum |
| Immunogen | Purified mouse IgG2b |
| Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
| Clones | VHH0275, VHH0288 |
| RRID | AB_2827581 |
| Affinity (KD) | CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM |
| Excitation / Emission | Excitation max: 490 nm, Emission max: 525 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
| Size | 10 µL; 100 µL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| sms2bAF488-1_SDS_Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 488 [CTK0105, CTK0106] (EN) |
| Datasheet |
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| Nano-Secondary® anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 488 [CTK0105, CTK0106] Datasheet |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
| Application note |
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| One-step Incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
Publications
| Application | Title |
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