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ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106]

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

Applications

IF, WB

Conjugate

Alexa Fluor® 568

Host

Alpaca

Reactivity

Mouse IgG2b

Cat No : sms2bAF568-1

Synonyms

mouse IgG2b; mouse immunoglobulin Gamma 2b

Validation Data Gallery

  • Anti-mouse IgG2b Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG2b antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG2b primary antibody.

    Anti-mouse IgG2b Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG2b antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG2b primary antibody.

  • HeLa cells were immunostained with mouse IgG2b anti-βTubulin antibody + alpaca anti-mouse IgG2b VHH Alexa Fluor® 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich

    HeLa cells were immunostained with mouse IgG2b anti-βTubulin antibody + alpaca anti-mouse IgG2b VHH Alexa Fluor® 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich

  • One-step immunostaining is the simultaneous incubation of mouse IgG2b primary antibody and anti-mouse IgG2b Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

    One-step immunostaining is the simultaneous incubation of mouse IgG2b primary antibody and anti-mouse IgG2b Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

  • Dissociation constant Kd and affinity.

    Dissociation constant Kd and affinity.

Product Information

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB
HostAlpaca
Species ReactivityMouse IgG2b Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3
ConjugateAlexa Fluor® 568
Physical StateLiquid
Suggested DilutionImmunofluorescence 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeMixture of 2 monoclonal Nanobodies
ClassRecombinant
Affinity (KD) CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
RRIDAB_2827582
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Science

Mechanism of spindle pole organization and instability in human oocytes.

Authors - Chun So
View Article