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ChromoTek anti-IFNy recombinant VHH, biotinylated

IFNg-b is a recombinant mono-biotinylated anti IFNg Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat No : IFNg-b

Synonyms

IFNg VHH, IFNg single domain antibody (sdAb), Interferon Gamma, Immune interferon, Interferon

Validation Data Gallery

  • BLI analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS).

    BLI analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS).

  • ELISA analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS). Detection via rabbit polyclonal PTG 15365-1-AP.

    ELISA analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS). Detection via rabbit polyclonal PTG 15365-1-AP.

Product Information

IFNg-b is a recombinant mono-biotinylated anti IFNg Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

ApplicationsBLI, SPR, ELISA
ReactivityHuman
ConjugateBiotin
TypeNanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.4 kDa
FormLiquid
RRIDAB_3665388
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingambient
BackgroundIFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).