验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | Customer Sample customer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ABAT Ready-To-Use IHC Kit. ABAT primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11349-1-AP targets ABAT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ABAT fusion protein Ag1877 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 4-aminobutyrate aminotransferase |
| 别名 | GABA AT, GABA aminotransferase, EC:2.6.1.22, EC:2.6.1.19, 4-aminobutyrate aminotransferase, mitochondrial |
| 计算分子量 | 500 aa, 56 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC015628 |
| 基因名称 | ABAT |
| Gene ID (NCBI) | 18 |
| RRID | AB_2220001 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P80404 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABAT antibody 11349-1-AP | Download protocol |
| IHC protocol for ABAT antibody 11349-1-AP | Download protocol |
| IP protocol for ABAT antibody 11349-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
Front Cell Neurosci Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA. | ||
Cancer Invest Integrative analysis developing and validating potential candidate biomarkers for cancer stemness features of pan-renal cell carcinoma | ||
Carcinogenesis EphB6 deficiency in intestinal neurons promotes tumor growth in colorectal cancer by neurotransmitter GABA signaling | ||
Cell Oncol (Dordr) TRPV1 inhibition suppresses non-small cell lung cancer progression by inhibiting tumour growth and enhancing the immune response | ||
MedComm (2020) Neurotransmitter accumulation and Parkinson's disease-like phenotype caused by anion channelrhodopsin opto-controlled astrocytic mitochondrial depolarization in substantia nigra pars compacta |