验证数据展示

WB analysis of mouse brain using 14139-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14139-1-AP
IP result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.
WB analysis using 14139-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 14139-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14139-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14139-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 14139-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADAM12 Polyclonal antibody (14139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14139-1-AP

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Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Synonyms

MLTN, Meltrin-alpha, MCMP, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 12

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (42)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, HeLa cells, mouse heart tissue, rat brain tissue, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human placenta tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 26 publications below
IHC	See 16 publications below
IF	See 8 publications below
IP	See 1 publications below
FC	See 2 publications below

产品信息

14139-1-AP targets ADAM12 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	ADAM12 fusion protein Ag5206 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ADAM metallopeptidase domain 12
Synonyms	MLTN, Meltrin-alpha, MCMP, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 12
Calculated Molecular Weight	100 kDa
Observed Molecular Weight	90-100 kDa, 70-80 kDa
GenBank Accession Number	BC060804
Gene Symbol	ADAM12
Gene ID (NCBI)	8038
RRID	AB_2289230
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43184
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

实验方案

Product Specific Protocols
WB protocol for ADAM12 antibody 14139-1-AP	Download protocol
IHC protocol for ADAM12 antibody 14139-1-AP	Download protocol
IF protocol for ADAM12 antibody 14139-1-AP	Download protocol
IP protocol for ADAM12 antibody 14139-1-AP	Download protocol
FC protocol for ADAM12 antibody 14139-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,human	IF	Cell Stem Cell Vaccination-based immunotherapy to target profibrotic cells in liver and lung Authors - Michal Sobecki View Article
human	IHC	J Cell Biol Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. Authors - Díaz Bego?a B View Article
human	IF	Theranostics Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. Authors - Yinan Chen View Article
human	WB,IF	Cell Death Differ ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion. Authors - M Aghababaei View Article
human	IHC	J Hematol Oncol Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway. Authors - Kosuke Ogawa View Article
human	WB,IHC	Oncogene A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion. Authors - Rao V H VH View Article

验证数据展示

WB analysis of mouse brain using 14139-1-AP

IP experiment of HeLa using 14139-1-AP

WB analysis using 14139-1-AP

IHC staining of human placenta using 14139-1-AP

IHC staining of human placenta using 14139-1-AP

IF Staining of HepG2 using 14139-1-AP

FC experiment of HeLa using 14139-1-AP

ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Vaccination-based immunotherapy to target profibrotic cells in liver and lung

Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.

Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.

Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.

A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion.