• Featured Product
  • KD/KO Validated

ATG5 Polyclonal antibody

ATG5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10181-2-AP

Print datasheet

Synonyms

Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse kidney tissue, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10181-2-AP targets ATG5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper
Immunogen ATG5 fusion protein Ag0214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Synonyms Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa, 40-45 kDa, 50-55 kDa
GenBank Accession NumberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
RRIDAB_2062045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

实验方案

Product Specific Protocols
WB protocol for ATG5 antibody 10181-2-APDownload protocol
IHC protocol for ATG5 antibody 10181-2-APDownload protocol
IP protocol for ATG5 antibody 10181-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Cell Res

Degradation of lipid droplets by chimeric autophagy-tethering compounds.

Authors - Yuhua Fu
  • KO Validated
humanWB

Mol Cell

Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.

Authors - Lu Yubing Y
  • KD Validated
humanWB

Adv Sci (Weinh)

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Authors - Xiaowei She
human

Autophagy

Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models

Authors - Panagiota Mavroeidi
mouseWB

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong