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Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
2212
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse spleen tissue, HeLa cells, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, mouse eye tissue |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19677-1-AP targets Caspase 3/p17/p19 in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, RIP, ELISA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, zebrafish, hamster, sheep, goat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Synonyms | CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32-35 kDa, 17 kDa, 19 kDa |
| GenBank Accession Number | NM_004346 |
| Gene Symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_10733244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
| IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
| IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
| IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Nat Microbiol Yersinia infection induces glucose depletion and AMPK-dependent inhibition of pyroptosis in mice | ||
Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection | ||
Nat Commun Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. |
