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验证数据展示

  • WB analysis of human plasma using 66109-1-Ig

    WB analysis of human plasma using 66109-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66109-1-Ig

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 66109-1-Ig

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

Clusterin Monoclonal antibody

Clusterin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66109-1-Ig

Print datasheet

CloneNo. 2F12G10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66109-1-Ig targets Clusterin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Clusterin fusion protein Ag19236 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name clusterin
Synonyms Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_2881508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
WB protocol for Clusterin antibody 66109-1-IgDownload protocol
IHC protocol for Clusterin antibody 66109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols