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- KD/KO Validated
COX5B Polyclonal antibody
COX5B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11418-2-AP targets COX5B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat, monkey |
| Immunogen | COX5B fusion protein Ag1986 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytochrome c oxidase subunit Vb |
| Synonyms | COX5B, COXVB |
| Calculated Molecular Weight | 129 aa, 14 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC006229 |
| Gene Symbol | COX5B |
| Gene ID (NCBI) | 1329 |
| RRID | AB_2245376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10606 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX5B antibody 11418-2-AP | Download protocol |
| IHC protocol for COX5B antibody 11418-2-AP | Download protocol |
| IF protocol for COX5B antibody 11418-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
| ||
Nat Immunol Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense. | ||
Cell Metab Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle. | ||
Mol Cell Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery |
