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验证数据展示

  • WB analysis of human spleen using 66701-1-Ig

    WB analysis of human spleen using 66701-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66701-1-Ig (CD21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD21 Monoclonal antibody

CD21 Monoclonal Antibody for WB, ELISA
Cat No. 66701-1-Ig

产品说明书

CloneNo. 1D9A3

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66701-1-Ig targets CD21 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 CD21 fusion protein Ag18058 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
计算分子量 1092 aa, 119 kDa
观测分子量 150 kDa
GenBank蛋白编号BC136394
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2882054
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20023
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
WB protocol for CD21 antibody 66701-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols