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验证数据展示

  • WB analysis of human spleen using 66701-1-Ig

    WB analysis of human spleen using 66701-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66701-1-Ig (CD21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD21 Monoclonal antibody

CD21 Monoclonal Antibody for WB, ELISA
Cat No. 66701-1-Ig

Print datasheet

CloneNo. 1D9A3

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66701-1-Ig targets CD21 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CD21 fusion protein Ag18058 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name complement component (3d/Epstein Barr virus) receptor 2
Synonyms C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
Calculated Molecular Weight 1092 aa, 119 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC136394
Gene Symbol CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2882054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
WB protocol for CD21 antibody 66701-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols