• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 10777-1-AP

    WB analysis using 10777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 10777-1-AP

    IHC staining of mouse brain using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10777-1-AP

    IHC staining of mouse brain using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SH-SY5Y using 10777-1-AP

    FC experiment of SH-SY5Y using 10777-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of rat brain using 10777-1-AP

    IF Staining of rat brain using 10777-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 10777-1-AP

    IF Staining of SH-SY5Y using 10777-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DBH Polyclonal antibody

DBH Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA
Cat No. 10777-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA and More (1)

别名

DBM, Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, EC:1.14.17.1, Soluble dopamine beta-hydroxylase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSH-SY5Y cells, NIH/3T3 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inrat brain tissue
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10777-1-AP targets DBH in WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag1196

Product name: Recombinant human DBH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 269-603 aa of BC017174

Sequence: CDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DBH
别名 DBM, Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, EC:1.14.17.1, Soluble dopamine beta-hydroxylase
计算分子量64.8 kDa
观测分子量 63 kDa
GenBank蛋白编号BC017174
基因名称 DBH
Gene ID (NCBI) 1621
RRIDAB_2277037
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09172
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).

实验方案

Product Specific Protocols
WB protocol for DBH antibody 10777-1-APDownload protocol
IHC protocol for DBH antibody 10777-1-APDownload protocol
IF protocol for DBH antibody 10777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.

Authors - Shin Takasawa
View Article
humanWB

Peptides

Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid.

Authors - Yuan-Jun Qin
View Article
mouseIF

Tissue Cell

Autonomic nervous system imbalance in diabetic mouse choroids

Authors - Yuan-Jun Qin
View Article