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- KD/KO Validated
E-cadherin Polyclonal antibody
E-cadherin Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF/ICC,IF-P, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
1723
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse colon tissue, A431 cells, mouse small intestine tissue, MCF-7 cells, mouse embryo tissue, human breast cancer tissue |
| Positive FC detected in | HepG2 cells |
Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20874-1-AP targets E-cadherin in WB,IP,IF,FC,IHC,CoIP,ELISA,Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC,IF-P, IHC, IP, WB, ELISA |
| Cited Applications | WB,IP,IF,FC,IHC,CoIP,Cell treatment |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, pig, canine, bovine |
| Immunogen | E-cadherin fusion protein Ag14973 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
| Synonyms | Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin |
| Calculated Molecular Weight | 882 aa, 97 kDa |
| Observed Molecular Weight | 120-125 kDa |
| GenBank Accession Number | BC141838 |
| Gene Symbol | CDH1 |
| Gene ID (NCBI) | 999 |
| RRID | AB_10697811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12830 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for E-cadherin antibody 20874-1-AP | Download protocol |
| IHC protocol for E-cadherin antibody 20874-1-AP | Download protocol |
| IF protocol for E-cadherin antibody 20874-1-AP | Download protocol |
| IP protocol for E-cadherin antibody 20874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice. | ||
Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 | ||
ACS Nano Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment. | ||
Nat Commun TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity | ||
Nat Commun UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis |
