验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21244-1-AP targets ER in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine, zebrafish, bovine, sheep, goat |
| 免疫原 |
CatNo: Ag15738 Product name: Recombinant human ER protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 65-280 aa of BC128573 Sequence: AAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | estrogen receptor 1 |
| 别名 | ESR1, ER alpha, Era, ER-alpha, ERα |
| 计算分子量 | 595 aa, 66 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC128573 |
| 基因名称 | ER |
| Gene ID (NCBI) | 2099 |
| RRID | AB_11042600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P03372 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ER antibody 21244-1-AP | Download protocol |
| IHC protocol for ER antibody 21244-1-AP | Download protocol |
| WB protocol for ER antibody 21244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Autonomously Motile Nano-PROTACs Act as Protein-Sweeping Robots to Enhance Targeted Protein Degradation. | ||
Drug Resist Updat TRIM24 promotes T-cell lymphoma development and glucocorticoid resistance via FUS-mediated phase separation of the glucocorticoid receptor | ||
Cell Discov Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals. | ||
Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII | ||
J Nanobiotechnology Exosome-delivered NR2F1-AS1 and NR2F1 drive phenotypic transition from dormancy to proliferation in treatment-resistant prostate cancer via stabilizing hormonal receptors | ||
Theranostics Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype |
