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  • KD/KO Validated

FURIN Polyclonal antibody

FURIN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18413-1-AP

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Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1



经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18413-1-AP targets FURIN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken
Immunogen FURIN fusion protein Ag13261 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name furin (paired basic amino acid cleaving enzyme)
Synonyms Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC012181
Gene Symbol FURIN
Gene ID (NCBI) 5045
RRIDAB_2878542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09958
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and the shed furin at 90 kDa.

实验方案

Product Specific Protocols
WB protocol for FURIN antibody 18413-1-APDownload protocol
IHC protocol for FURIN antibody 18413-1-APDownload protocol
IP protocol for FURIN antibody 18413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Rep

Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury

Authors - Yiheng Mao
humanWB

Oncogene

Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.

Authors - Diana Farhat
humanWB

PLoS Pathog

Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2

Authors - Shi Yu
  • KD Validated
humanWB

Oncogenesis

Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.

Authors - Huizhi Wang
humanWB

Br J Cancer

Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.

Authors - Diana Farhat
  • KD Validated
humanWB,IF

J Virol

Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry

Authors - Timothy J Smith
  • KO Validated