Speedy™ Human/Cynomolgus Monkey G-CSF One-Step ELISA Kit
Sensitivity
11.0 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human/Cynomolgus Monkey
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey G-CSF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1440 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 77% | 71%-81% |
| Human cell culture supernatant | 100% | 93%-110% |
| Cynomolgus monkey cell culture supernatant | 100% | 95%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 975.6 | 21.1 | 2.2 |
| 2 | 8 | 224.3 | 4.9 | 2.2 |
| 3 | 8 | 112.8 | 3.3 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 968.2 | 18.1 | 1.9 |
| 2 | 16 | 222.2 | 4.5 | 2.0 |
| 3 | 16 | 111.5 | 3.1 | 2.8 |
Background Information
Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSF3, C17orf33, CSF 3, FilgrastimLenograstim, GCSF |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
