Speedy™ Human IgG4 One-Step ELISA Kit
Sensitivity
2.5 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IgG4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Urine,Saliva,Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3503 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 89%-108% |
| Urine | 100% | 95%-107% |
| Saliva | 94% | 92%-97% |
| Human milk | 90% | 75%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,629.7 | 77.3 | 2.9 |
| 2 | 8 | 670.0 | 15.0 | 2.2 |
| 3 | 8 | 335.0 | 8.8 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,665.3 | 83.0 | 3.1 |
| 2 | 16 | 659.4 | 19.8 | 3.0 |
| 3 | 16 | 338.4 | 9.8 | 2.9 |
Background Information
Immunoglobulin G4 (IgG4) is the least abundant subclass of immunoglobulin G, comprising only 3-6% of total serum IgG. It exhibits unique structural properties, including Fab-arm exchange and asymmetric half-molecules, resulting in monovalent binding and anti-inflammatory functions. Unlike other IgG subclasses, IgG4 does not effectively activate complement or engage Fc receptors, classifying it as a "blocking antibody" that can dampen immune responses. Clinically, elevated IgG4 levels are pathognomonic for IgG4-related disease (IgG4-RD), a fibroinflammatory condition characterized by tumefactive lesions, dense lymphoplasmacytic infiltrates rich in IgG4-positive plasma cells, and storiform fibrosis. First recognized in autoimmune pancreatitis, IgG4-RD now encompasses multiple organ systems including the biliary tract, retroperitoneum, salivary glands, and kidneys.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | human IgG heavy chain, Human IgG4, IgG Fc, IGHG, IGHG4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
