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IFITM1 Polyclonal antibody

IFITM1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

FC, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11727-3-AP

Print datasheet

Synonyms

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13



经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inROS1728 cells
Positive IHC detected inhuman lymphoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11727-3-AP targets IFITM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC, IHC, IP, WB,ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, goat, African green monkey
Immunogen IFITM1 fusion protein Ag2320 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon induced transmembrane protein 1 (9-27)
Synonyms 9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC000897
Gene Symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2122083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

实验方案

Product Specific Protocols
WB protocol for IFITM1 antibody 11727-3-APDownload protocol
IHC protocol for IFITM1 antibody 11727-3-APDownload protocol
IP protocol for IFITM1 antibody 11727-3-APDownload protocol
FC protocol for IFITM1 antibody 11727-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Mitotic progression following DNA damage enables pattern recognition within micronuclei.

Authors - Shane M Harding
humanFC

Nature

Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.

Authors - Edward J Grow
  • KD Validated
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanWB

Mol Ther

Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.

Authors - Sarah Aref
humanWB

Nat Commun

IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses

Authors - M. Clement
humanWB

Cell Rep

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Authors - Jingyou Yu
  • KD Validated