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NeutraKine® IFN-gamma Monoclonal antibody

NeutraKine® IFN-gamma Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

1E10G7

Cat no : 69007-1-Ig

Print datasheet

Synonyms

NeutraKine® IFN Gamma, IFNG, IFN-gamma, IFN γ, IFN gamma

验证数据展示

  • Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

    Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (2ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL.

  • Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

    Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using 69007-1-Ig

    ELISA experiment of Recombinant protein using 69007-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
NeutralizationHT-29 cell

产品信息

69007-1-Ig targets NeutraKine® IFN-gamma in Neutralization, ELISA applications and shows reactivity with human samples.

Tested Applications Neutralization, ELISA Application Description
Cited ApplicationsNeutralization
Tested Reactivity human
Immunogen human Humankine IFN-gamma protein HZ-1301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IFN gamma
Synonyms NeutraKine® IFN Gamma, IFNG, IFN-gamma, IFN γ, IFN gamma
Gene Symbol IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_2882925
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.

发表文章

SpeciesApplicationTitle
Neutralization

Cell Mol Gastroenterol Hepatol

Fatty Acid Oxidation Promotes Apoptotic Resistance and Proinflammatory Phenotype of CD4+ Tissue-resident Memory T cells in Crohn's Disease

Authors - Guanzhan Liang
View Article