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Integrin Alpha 4 Polyclonal antibody
Integrin Alpha 4 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19676-1-AP targets Integrin Alpha 4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, WB, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Synonyms | CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_000885 |
| Gene Symbol | ITGA4 |
| Gene ID (NCBI) | 3676 |
| RRID | AB_10640907 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha 4 antibody 19676-1-AP | Download protocol |
| IHC protocol for Integrin Alpha 4 antibody 19676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. | ||
J Exp Med EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model. | ||
Int J Biol Macromol Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis | ||
Int J Mol Sci Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury. | ||
Int J Mol Sci Sesquiterpene Lactone Deoxyelephantopin Isolated from Elephantopus scaber and Its Derivative DETD-35 Suppress BRAFV600E Mutant Melanoma Lung Metastasis in Mice. | ||
J Neuroinflammation Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke. |
