验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, A549 cells, MCF-7 cells, human placenta tissue |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27096-1-AP targets Integrin Alpha V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Integrin Alpha V fusion protein Ag25825 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) |
| 别名 | ITGAV, MSK8, Integrin alpha-V light chain, Integrin alpha-V heavy chain, Integrin alpha-V |
| 计算分子量 | 1048 aa, 116 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC136442 |
| 基因名称 | Integrin alpha V |
| Gene ID (NCBI) | 3685 |
| RRID | AB_2880753 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06756 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha V antibody 27096-1-AP | Download protocol |
| IHC protocol for Integrin Alpha V antibody 27096-1-AP | Download protocol |
| IF protocol for Integrin Alpha V antibody 27096-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.
| ||
Curr Biol The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface. | ||
Int J Mol Sci Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression. | ||
Cell Signal C1GALT1 high expression enhances the progression of glioblastoma through the EGFR-AKT/ERK cascade | ||
Int J Mol Sci Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients. |
