• Featured Product
  • KD/KO验证

KU70,XRCC6 Polyclonal antibody

KU70,XRCC6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10723-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

KU70, XRCC6, CTC75, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman renal cell carcinoma tissue, human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10723-1-AP targets KU70,XRCC6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 KU70,XRCC6 fusion protein Ag1043 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 6
别名 KU70, XRCC6, CTC75, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit
计算分子量 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC008343
基因名称 KU70
Gene ID (NCBI) 2547
RRIDAB_2218756
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12956
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

实验方案

Product Specific Protocols
WB protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IHC protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IF protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
IP protocol for KU70,XRCC6 antibody 10723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
humanWB,IF

Oncogene

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Authors - L Hu
humanWB

Phytomedicine

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer

Authors - Meiyang Chen
humanWB

J Mater Chem B

Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer

Authors - Shuying Li
humanWB

FEBS J

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy

Authors - Yan Wei
ratWB

FASEB J

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Authors - Catherine E Aiken
Loading...