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PAX8 Polyclonal antibody
PAX8 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
414
验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells |
| Positive IHC detected in | human ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SKOV-3 cells, mouse kidney tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:5000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10336-1-AP targets PAX8 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, IHC, WB, ELISA |
| Cited Applications | CoIP, FC, IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | fusion protein |
| Full Name | paired box 8 |
| Synonyms | paired box 8, Paired box protein Pax 8, PAX8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa, 58 kDa |
| GenBank accession number | BC001060 |
| Gene symbol | PAX8 |
| Uniprot | Q06710 | Gene ID (NCBI) | 7849 |
| RRID | AB_2236705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IHC protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IF protocol for PAX8 antibody 10336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virchows Arch Histological and molecular characterization of TFEB-rearranged renal cell carcinomas. | ||
Commun Biol A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids. | ||
Hum Pathol MDM2 amplification and immunohistochemical expression in sarcomatoid renal cell carcinoma. | ||
Nat Mater Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells. | ||
Reprod Sci IFITM1 is a Novel, Highly Sensitive Marker for Endometriotic Stromal Cells in Ovarian and Extragenital Endometriosis. |
