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PAX8 Polyclonal antibody
PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
516
验证数据展示
经过测试的应用
Positive WB detected in | SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells |
Positive IP detected in | SKOV-3 cells, HeLa cells |
Positive IHC detected in | human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse kidney tissue |
Positive IF/ICC detected in | SKOV-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP, ELISA |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig, rabbit, zebrafish, bovine |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | paired box 8 |
Synonyms | PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa, 58 kDa |
GenBank Accession Number | BC001060 |
Gene Symbol | PAX8 |
Gene ID (NCBI) | 7849 |
RRID | AB_2236705 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q06710 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
实验方案
Product Specific Protocols | |
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WB protocol for PAX8 antibody 10336-1-AP | Download protocol |
IHC protocol for PAX8 antibody 10336-1-AP | Download protocol |
IF protocol for PAX8 antibody 10336-1-AP | Download protocol |
IP protocol for PAX8 antibody 10336-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.
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Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Med Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice. | ||
Cancer Discov A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma. | ||
Cell Stem Cell Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. | ||
Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. |