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  • KD/KO Validated

PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10336-1-AP

Print datasheet

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8



经过测试的应用

Positive WB detected inSKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells
Positive IP detected inSKOV-3 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, ELISA
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, rabbit, zebrafish, bovine
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paired box 8
Synonyms PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa, 58 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_2236705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06710
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

实验方案

Product Specific Protocols
WB protocol for PAX8 antibody 10336-1-APDownload protocol
IHC protocol for PAX8 antibody 10336-1-APDownload protocol
IF protocol for PAX8 antibody 10336-1-APDownload protocol
IP protocol for PAX8 antibody 10336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nature

The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.

Authors - Saroor A Patel
  • KD Validated
humanIHC

Nat Biotechnol

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Authors - Frans Schutgens
humanIHC

Nat Med

Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice.

Authors - Sabine Harlander
human,mouseWB,IHC

Cancer Discov

A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma.

Authors - Phillip M Rappold
humanIHC

Cell Stem Cell

Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer.

Authors - Kadi Lõhmussaar
humanIF

Cell Stem Cell

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.

Authors - Jian Hui Low