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PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10336-1-AP

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Synonyms

paired box 8, Paired box protein Pax 8, PAX8



经过测试的应用

Positive WB detected inSKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells
Positive IHC detected inhuman ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

10336-1-AP targets PAX8 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa, 58 kDa
GenBank accession numberBC001060
Gene symbol PAX8
UniprotQ06710
Gene ID (NCBI) 7849
RRIDAB_2236705
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

实验方案

Product Specific Protocols
WB protocol for PAX8 antibody 10336-1-APDownload protocol
IHC protocol for PAX8 antibody 10336-1-APDownload protocol
IF protocol for PAX8 antibody 10336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Virchows Arch

Histological and molecular characterization of TFEB-rearranged renal cell carcinomas.

Authors - Nicolas Wyvekens
humanIHC

Commun Biol

A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids.

Authors - Nhan Phan
humanIHC

Hum Pathol

MDM2 amplification and immunohistochemical expression in sarcomatoid renal cell carcinoma.

Authors - David Suster
humanIHC

Reprod Sci

PAX8: A Highly Sensitive Marker for the Glands in Extragenital Endometriosis.

Authors - Tomoko Arakawa
humanIF

Nat Mater

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells.

Authors - Elena Garreta
humanIHC

Reprod Sci

IFITM1 is a Novel, Highly Sensitive Marker for Endometriotic Stromal Cells in Ovarian and Extragenital Endometriosis.

Authors - Hui Sun