验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells |
| Positive IP detected in | SKOV-3 cells, HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, IHC-P-IF applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, ELISA, IHC-P-IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, zebrafish, bovine |
| 免疫原 | fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | paired box 8 |
| 别名 | PAX-8, paired box 8, Paired box protein Pax 8, Paired box protein Pax-8, PAX 8 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa, 58 kDa |
| GenBank蛋白编号 | BC001060 |
| 基因名称 | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_2236705 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06710 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IHC protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IF protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IP protocol for PAX8 antibody 10336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.
| ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Med Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice. | ||
Cancer Discov A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma. | ||
Cell Stem Cell Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. | ||
Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. |
