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PAX8 Polyclonal antibody 378 Publications

Rabbit Polyclonal| Catalog number: 10336-1-AP

Featured Product KD/KO validated

浓度: 500 μg/ml

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Applications

Tested Applications:
IF, IHC, WB,ELISA
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Cited Applications:
FC, IF, IHC, WB


Images See all

WB analysis of SKOV-3 using 10336-1-AP

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IHC staining of human ovary tumor using 10336-1-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10336-1-AP (PAX8 Antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human ovary tumor using 10336-1-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10336-1-AP (PAX8 Antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of SKOV-3 using 10336-1-AP

Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 10336-1-AP (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidin stains F-actin (Red).

IF Staining of mouse kidney using 10336-1-AP

Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 10336-1-AP (PAX8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Species Specificity:
human, mouse, rat
Cited Species:
bovine, human, mouse, pig, rat, zebrafish
Positive WB detected in:
SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells
Positive IHC detected in:
human ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SKOV-3 cells, mouse kidney tissue
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:1000-1:5000
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PAX8 fusion protein Ag0306
Full name:
paired box 8
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa, 58 kDa
GenBank accession number:
BC001060
Gene ID (NCBI):
7849
Gene symbol
PAX8
Synonyms
paired box 8, Paired box protein Pax 8, PAX8

Background

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Protocols

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