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  • KD/KO Validated

POLD3 Polyclonal antibody

POLD3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21935-1-AP

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Synonyms

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3



经过测试的应用

Positive WB detected inMCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21935-1-AP is POLD3 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen POLD3 fusion protein Ag16570 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA-directed), delta 3, accessory subunit
Synonyms DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3
Calculated Molecular Weight 466 aa, 51 kDa
Observed Molecular Weight 51-70 kDa
GenBank Accession NumberBC108908
Gene Symbol POLD3
Gene ID (NCBI) 10714
RRIDAB_2878949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15054
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLD3 antibody 21935-1-APDownload protocol
IHC protocol for POLD3 antibody 21935-1-APDownload protocol
IP protocol for POLD3 antibody 21935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

Break-induced replication promotes fragile telomere formation.

Authors - Zhe Yang
  • KO Validated
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
  • KD Validated
humanWB

J Adv Res

Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA

Authors - Xi Dong
human

Mol Cell

Mechanisms of insertions at a DNA double-strand break

Authors - Jaewon Min