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SARS-COV-2 NSP5 Polyclonal antibody

SARS-COV-2 NSP5 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus and More (1)

Applications

IF, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 29286-1-AP

Print datasheet

Synonyms

SARS-COV-2 NSP5

验证数据展示

  • IF Staining of HEK-293T using 29286-1-AP

    IF Staining of HEK-293T using 29286-1-AP

    Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

  • ELISA experiment using 29286-1-AP

    ELISA experiment using 29286-1-AP

    SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive IF detected inHEK-293T cells
Positive ELISA detected inN/A

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29286-1-AP targets SARS-COV-2 NSP5 in WB, IF, ELISA applications and shows reactivity with virus samples.

Tested Applications IF, ELISA
Cited Applications WB
Tested Reactivity virus
Cited Reactivityhuman
Immunogen SARS-COV-2 NSP5 fusion protein Ag30934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ORF1ab
Synonyms SARS-COV-2 NSP5
GenBank Accession NumberYP_009742612
Gene Symbol ORF1ab
Gene ID (NCBI) 43740578
RRIDAB_2918269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article