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SCRIB Polyclonal antibody

SCRIB Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat No : 27083-1-AP

Print datasheet

Synonyms

CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB, SCRIB1, Scribble, scribbled homolog (Drosophila), Vartul

验证数据展示

  • WB analysis using 27083-1-AP

    WB analysis using 27083-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27083-1-AP (SCRIB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 27083-1-AP is SCRIB Fusion Protein expressed in E. coli.

Tested Applications WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SCRIB fusion protein Ag25795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name scribbled homolog (Drosophila)
Synonyms CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB, SCRIB1, Scribble, scribbled homolog (Drosophila), Vartul
Calculated Molecular Weight 1630 aa, 175 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC036905
Gene Symbol SCRIB
Gene ID (NCBI) 23513
RRIDAB_2880749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRIB encodes a large, 260-kDa cytoplasmic scaffolding protein that comprises a large leucine-rich repeat (LRR) region and 4 PDZ domains that regulate protein-protein interactions. In mammals, cell polarity is established and maintained by at least 3 protein modules (Scrib, Crumbs, and Par). The apical (Crumbs and Par) and basolateral (Scrib) modules function in a mutually antagonistic relationship to regulate various polarization processes such as apical-basal polarity, planar cell polarity, asymmetric cell division and migration. The loss of SCRIB in malignant tumors suggest that it has potential to be a tumor suppressor. Alteration of polarity through deletion, downregulation, overexpression, and mislocalization can induce structural and functional alteration of cells that might be related to tumorigenesis. Mislocalization of SCRIB is involved in epithelial-to-mesenchymal transition (EMT) by inducing loss of E-cadherin. Deregulated SCRIB expression induces tumorigenesis in breast and liver. (PMID: 28460446, PMID: 32564009)

实验方案

Product Specific Protocols
WB protocol for SCRIB antibody 27083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

MiR-9-5p Inhibits the MMP+-Induced Neuron Apoptosis through Regulating SCRIB/β-Catenin Signaling in Parkinson's Disease.

Authors - Zhenyong Xiao
View Article