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  • KD/KO Validated

TMED9 Polyclonal antibody

TMED9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21620-1-AP

Print datasheet

Synonyms

Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9



经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21620-1-AP targets TMED9 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen TMED9 fusion protein Ag13813 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane emp24 protein transport domain containing 9
Synonyms Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9
Calculated Molecular Weight 235 aa, 27 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC001123
Gene Symbol TMED9
Gene ID (NCBI) 54732
RRIDAB_10858623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

实验方案

Product Specific Protocols
WB protocol for TMED9 antibody 21620-1-APDownload protocol
IHC protocol for TMED9 antibody 21620-1-APDownload protocol
IF protocol for TMED9 antibody 21620-1-APDownload protocol
IP protocol for TMED9 antibody 21620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated
humanWB,CoIP

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
humanWB

BMC Biol

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Authors - Xihua Yue
humanWB,IHC

Bioengineered

High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.

Authors - Gaoda Ju
  • KD Validated
humanWB

Adv Sci (Weinh)

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Authors - Hak Park