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Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10094-1-AP

Print datasheet

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

验证数据展示

  • WB analysis using 10094-1-AP

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (Beta Tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10094-1-AP

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10094-1-AP

    IP experiment of mouse brain using 10094-1-AP

    IP result of anti-Beta Tubulin (IP:10094-1-AP, 3ug; Detection:10094-1-AP 1:1000) with mouse brain tissue lysate 7500ug.

  • IHC staining of human colon using 10094-1-AP

    IHC staining of human colon using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (Beta Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human colon using 10094-1-AP

    IHC staining of human colon using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (Beta Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IF Staining of mouse eye using 10094-1-AP

    IF Staining of mouse eye using 10094-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (10094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10094-1-AP

    IF Staining of HeLa using 10094-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (10094-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inU-251 cells, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human cerebellum tissue, mouse brain tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10094-1-AP targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, chicken, zebrafish, hamster, sheep, goat, fish
Immunogen Beta Tubulin fusion protein Ag0136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2210695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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